On January 7, 2021, in accordance with the relevant regulations of the "Administrative Measures for the Recognition of High-tech Enterprises" (Guoke Fahuo [2016] No. 32) and the "Guidelines for the Management of the Recognition of High-tech Enterprises" (Guoke Fahuo [2016] No. 195)),Beijing Enze Kangtai Biotechnology Co., Ltd. relies on the self-developed and produced high-purity & high-recovery exosome isolation and purification kit-ExosupurTM and high-accuracy, low-input exosome library construction and quality control technology, the original organization Exosome research technology, exosome mass production and transformation technology, as well as the company's good sense of social responsibility, have been successfully identified as a high-tech enterprise through procedures such as enterprise declaration, expert review, and local review and recommendation!

　　For this selection, the invested company Enze Kangtai said: Innovation is the soul of enterprise development. Relying on the values of professional rigor, sharing, collaboration, innovation and efficiency, Enze Kangtai provides clinical customers with overall solutions, empowers clinical research, and accelerates clinical transformation. In order to ultimately promote the development of medicine and benefit a better life! The selection of high-tech enterprises this time is the recognition of Enze Kangtai by the government and society. The rapid development of Enze Kangtai is inseparable from the trust and support of our customers. More and better products and solutions!
　　In addition, in March 2021, six departments, including the Beijing Municipal Science and Technology Commission, the Beijing Municipal Development and Reform Commission, the Beijing Municipal Bureau of Economy and Information Technology, the Beijing Municipal Housing and Urban-rural Development Commission, the Beijing Municipal Administration for Market Supervision, and the Zhongguancun Science and Technology Park Management Committee, jointly released the 2020 Third Annual Report. Approved list of new technologies and new products (services) in Beijing (the 14th batch in total). Self-developed by Beijing Enze Kangtai Biotechnology Co., Ltd.Exosome purification kit Exosupur, exosome proteomics service, exosome functional service, tissue exosome service and exosome RNA omics service (including qPCR verification) 5 exosome products are included. .
　　"Beijing New Technology and New Product (Service) Certification" is jointly identified by six units including Beijing Municipal Science and Technology Commission. Review, and finally propose a list of new technologies and new products (services), which aims to promote the development of high-tech industries, promote the application of new technologies and new products (services), provide impetus for the improvement of enterprise innovation and scientific research capabilities, and guide enterprises to serve economic development. , urban construction and improvement of people's livelihood, with high authority.

　　Exosome purification kit Exosupur Based on the principle of SEC + ultrafiltration, the process and device are further optimized, so that the recovery rate of exosomes from plasma samples with complex components is as high as 95%, and 99.5% of plasma free impurities can be effectively removed. It is the best choice for exosome researchers. In addition, the Exosupur kit only recovers a fixed size range, and the product has a narrow particle size range, retaining the complete shape and function of exosomes, and is also a good assistant for functional research.
　　Exosomal Proteomics Services Exosomes contain abundant proteins, including exosome own proteins and proteins of source cells. Due to the small size of exosomes, the strong penetrability can pass through the body tissue barrier, the proteins in exosomes can be protected from the degradation of enzymes in the blood circulation, and the plasma exosome proteins are widely involved in the occurrence and development of various diseases and other factors. Exosomal proteins are emerging as novel markers for disease monitoring and efficacy assessment, including cancer.

　　Exosome functional services The first overall service from cell culture, exosome mass production and transformation to exosome functional mechanism research; effectively solve the problem of exosome mass production - difficulties and key points in exosome function research; exosome + cell Functional verification + animal in vivo verification + mechanism elaboration, the symbol of high-scoring articles, is also a must for the application of national natural subjects; the same gene can target and regulate functional pathways in different directions, and the same subject can derive multiple research directions; The mechanism period is 3-5 months; adhere to the authenticity, objectivity and traceability of all data, and provide result charts that can be directly used for article publication; provide privately customized technical routes according to scientific issues and cell types.

　　Tissue Exosome Services At present, the research methods for obtaining exosomes from tissues are divided into two categories: the exosomes in the interstitial space are directly isolated from the isolated tissue, that is, we usually refer to "tissue exosomes"; the other is through The tissue is cultured in vitro, and exosomes are isolated from the cultured supernatant, which is the "explant" in the mouths of the bigwigs. This research method of explants has been studied as early as 2009 in the journal Diabetes. The researchers isolated exosomes from the supernatant after culturing the adipose tissue <4mm isolated from mice in DMEM medium for a period of time. .

　　Exosome RNAomics service (including qPCR verification) ExosupurThe kit can obtain high-purity exosomes and ensure the accuracy of whole transcriptome sequencing from the source; it supports the input amount of sample RNA as low as 1ng; the UMI+ ultra-trace chain-specific library construction strategy can accurately restore miRNA, long-term The original state of the strand RNA, and improve the consistency with the verification results; from exosome isolation, RNA extraction to library construction and sequencing, quality control is carried out at every level to ensure the output of high-quality data; privately customized project plans , providing one-stop exosome whole transcriptome research solutions.

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